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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases

Anti-PKD2 antibody (ab245528)

Anti-PKD2 antibody (ab245528)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PKD2
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PKD2 antibody
    See all PKD2 primary antibodies
  • Description

    Rabbit polyclonal to PKD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PKD2 aa 825-878. The exact sequence is proprietary. NP_057541.2
    Database link: Q9BZL6

  • Positive control

    • WB: HeLa, HEK-293 and HEK-293T whole cell lysates. IP: HEK-293 whole cell lysate. IHC-P: Human stomach carcinoma tissue.
  • General notes

     This product was previously labelled as Protein Kinase D2

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245528 was affinity purified using an epitope specific to PKD2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKD2 antibody (ab245528)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKD2 antibody (ab245528)

    Formalin-fixed, paraffin-embedded human stomach carcinoma tissue stained for PKD2 using ab245528 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.

  • Western blot - Anti-PKD2 antibody (ab245528)
    Western blot - Anti-PKD2 antibody (ab245528)
    All lanes : Anti-PKD2 antibody (ab245528) at 0.2 µg

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 97 kDa


    Exposure time: 3 minutes


    Lysates prepared in NETN buffer.

  • Western blot - Anti-PKD2 antibody (ab245528)
    Western blot - Anti-PKD2 antibody (ab245528)
    Lanes 1-4 : Anti-PKD2 antibody (ab245528) at 2 µg/ml
    Lanes 5-8 : Anti-PKD2 antibody (ab245528) at 1 µg/ml

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lanes 2 & 6 : HEK-293 (transfected with PKD3-GFP) whole cell lysate
    Lanes 3 & 7 : HEK-293 (transfected with PKD1-GFP) whole cell lysate
    Lanes 4 & 8 : HEK-293 (transfected with GST-PKD3) whole cell lysate
    Lane 5 : HEK-293 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 97 kDa


    Exposure time: 10 seconds


    PKD2 homologues were co-expressed to assess cross-reactivity.

  • Immunoprecipitation - Anti-PKD2 antibody (ab245528)
    Immunoprecipitation - Anti-PKD2 antibody (ab245528)

    PKD2 was immunoprecipitated from HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate with ab245528 at 10 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245528 at 1 µg/ml.

    Lane 1: Control IgG IP in HEK-293 whole cell lysate.
    Lane 2: ab245528 IP in HEK-293 whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PKD2 antibody (ab245528)

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    Anti-PKD2 antibody [EP1495Y] (ab51250)

    Applications: ICC/IF, IHC-P, WB

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