Anti-Bax antibody (ab53154)
Key features and details
- Rabbit polyclonal to Bax
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Bax antibody
See all Bax primary antibodies -
Description
Rabbit polyclonal to Bax -
Host species
Rabbit -
Specificity
Preliminary customer data suggests that the current lot may not work in rat species, therefore it will be removed as a validated species until new testing is available.
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Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Human Bax. The immunogen in within aa 1-50.
Database link: Q07812
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Bax antibody (ab53154)All lanes : Anti-Bax antibody (ab53154) at 1/500 dilution
Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts with immunizing peptide
Predicted band size: 21 kDa
Observed band size: 21 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Bax antibody (ab53154)Immunofluorescence analysis of A549 (Human lung carcinoma cell line) cells, using ab53154 at 1/100 dilution. The picture on the right is treated with the synthesized peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody (ab53154)Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using ab53154 at 1/50 dilution. The picture on the right is treated with the synthesized peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody (ab53154)ab53154 staining human normal gallbladder tissue. Staining is localized to cell membrane and cytoplasm.
Left panel: ab53154 at 4 µg/ml. Right panel: Isotype control.
Sections were stained using an automated system at room temperature. Sections were rehydrated and antigen retrieved with the EDTA pH 9.0. Slides were blocked in 3% H2O2, methanol for 10 minutes. They were then blocked for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with hematoxylin and coverslipped. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
