Anti-PHPT1 antibody [001] - BSA and Azide free (ab276060)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [001] to PHPT1 - BSA and Azide free
- Suitable for: Flow Cyt, ICC, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-PHPT1 antibody [001] - BSA and Azide free
See all PHPT1 primary antibodies -
Description
Rabbit monoclonal [001] to PHPT1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PHPT1 aa 2-125.
Database link: Q9NRX4-1 -
Positive control
- IHC-P: Human skeletal muscle tissue. Flow cyt: THP1 cells. ICC: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
001 -
Isotype
IgG
Images
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Immunofluorescence staining of Human PHPT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab276060 at 1/60 dilution at 37ºC 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
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Flow cytometric analysis of Human PHPT1 expression on THP1 cells. The cells were treated according to manufacturers manuall, stained with ab276060, then a FITC-conjugated secondary antibody (black line) compared with a negative control (grey line). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Immunochemical staining of human PHPT1 in human skeletal muscle with ab276060 at 1/5000 dilution, formalin-fixed paraffin embedded sections.
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