Anti-DARPP32 (phospho T75) antibody (ab51114)
Key features and details
- Rabbit polyclonal to DARPP32 (phospho T75)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DARPP32 (phospho T75) antibody
See all DARPP32 primary antibodies -
Description
Rabbit polyclonal to DARPP32 (phospho T75) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of DARPP32 only when phosphorylated at threonine 75. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from human DARPP32 around the phosphorylation site of threonine 75 (A-Y-phosphoT-P-P).
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Positive control
- Human brain tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 (phospho T75) antibody (ab51114)Ab51114 staining human DARPP32 in human brain tissue by immunohistochemistry using paraffin embedded tissue.
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Western blot - Anti-DARPP32 (phospho T75) antibody (ab51114)All lanes : Anti-DARPP32 (phospho T75) antibody (ab51114) at 1 µg/ml
Lane 1 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 32 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
