Anti-MKRN1 antibody (ab123804)
Key features and details
- Goat polyclonal to MKRN1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MKRN1 antibody
See all MKRN1 primary antibodies -
Description
Goat polyclonal to MKRN1 -
Host species
Goat -
Specificity
ab123804 is expected to recognize both reported isoforms (NP_038474.2; NP_001138597.1). -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide:
RYEHSKPLKQEEAT
by a Cysteine residue linker, corresponding to internal sequence amino acids 105-118 of Human MKRN1 (NP_038474.2; NP_001138597.1). -
Positive control
- MKRN1 transfected HEK293 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab123804 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MKRN1 antibody (ab123804)All lanes : Anti-MKRN1 antibody (ab123804) at 1 µg/ml
Lane 1 : fetal mouse brain tissue lysate
Lane 2 : adult mouse brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 53 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa (possible isoform)
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Western blot - Anti-MKRN1 antibody (ab123804)Lane 1 : Anti-MKRN1 antibody (ab123804) at 0.3 µg/ml
Lane 2 : Anti-MKRN1 antibody (ab123804) at 1 mg/ml
Lane 3 : anti-DYKDDDDK Tag anntibody at 1/1000 dilution
Lane 1 : HEK293 lysate, over-expressing Human MKRN1 with DYKDDDDK tag in RIPA buffer
Lane 2 : HEK293 lysate, mock-transfected
Lane 3 : HEK293 lysate, over-expressing Human MKRN1 with DYKDDDDK tag in RIPA buffer
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
