Anti-TBX5 antibody [EPR23798-102] - BSA and Azide free (ab281835)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23798-102] to TBX5 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-TBX5 antibody [EPR23798-102] - BSA and Azide free
See all TBX5 primary antibodies -
Description
Rabbit monoclonal [EPR23798-102] to TBX5 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra),ICC,IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse P0 heart, Mouse E18 heart, and Mouse E17 heart lysates.
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General notes
ab281835 is the carrier-free version of ab259980. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab281835 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23798-102 -
Isotype
IgG
Images
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All lanes : Anti-TBX5 antibody [EPR23798-102] (ab259980) at 1/1000 dilution
Lane 1 : Mouse P0 heart tissue lysate
Lane 2 : Mouse P0 liver tissue lysate
Lane 3 : Mouse E18 heart tissue lysate
Lane 4 : Mouse E18 liver tissue lysate
Lane 5 : Mouse E17 heart tissue lysate
Lane 6 : Mouse E17 liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))
Predicted band size: 58 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?This data was developed using 259980, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: mouse embryo/fetal liver (PIMID:16894625).
Exposure time: 70 seconds
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