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Anti-TBX5 antibody [EPR23798-102] - BSA and Azide free (ab281835)

Price and availability

526 012 ₸

Availability

Order now and get it on Wednesday March 31, 2021

Anti-TBX5 antibody [EPR23798-102] - BSA and Azide free (ab281835)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23798-102] to TBX5 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-TBX5 antibody [EPR23798-102] - BSA and Azide free
    See all TBX5 primary antibodies
  • Description

    Rabbit monoclonal [EPR23798-102] to TBX5 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt (Intra),ICC,IHC-Fr,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse P0 heart, Mouse E18 heart, and Mouse E17 heart lysates.
  • General notes

    ab281835 is the carrier-free version of ab259980. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab281835 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituent: 100% PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23798-102
  • Isotype

    IgG

Images

  • Western blot - Anti-TBX5 antibody [EPR23798-102] - BSA and Azide free (ab281835)
    Western blot - Anti-TBX5 antibody [EPR23798-102] - BSA and Azide free (ab281835)
    All lanes : Anti-TBX5 antibody [EPR23798-102] (ab259980) at 1/1000 dilution

    Lane 1 : Mouse P0 heart tissue lysate
    Lane 2 : Mouse P0 liver tissue lysate
    Lane 3 : Mouse E18 heart tissue lysate
    Lane 4 : Mouse E18 liver tissue lysate
    Lane 5 : Mouse E17 heart tissue lysate
    Lane 6 : Mouse E17 liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 58 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?



    This data was developed using 259980, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: mouse embryo/fetal liver (PIMID:16894625).

    Exposure time: 70 seconds

     

  • Anti-TBX5 antibody [EPR23798-102] - BSA and Azide free (ab281835)
    Anti-TBX5 antibody [EPR23798-102] - BSA and Azide free (ab281835)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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