Formaldehyde Assay Kit (Fluorometric) (ab196997)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Adherent cells, Other biological fluids, Plasma, Saliva, Serum, Suspension cells, Tissue, Urine
- Sensitivity: 2 µM
Overview
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Product name
Formaldehyde Assay Kit (Fluorometric)
See all Formaldehyde kits -
Detection method
Fluorescent -
Sample type
Saliva, Urine, Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
> 2 µM -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Formaldehyde Assay Kit (Fluorometric) (ab196997) provides a simple, sensitive and high-throughput adaptable assay that detects biologically relevant concentrations of formaldehyde (HCHO) in various fluids and tissues. The assay relies on the oxidation of formaldehyde to produce a stable fluorescent signal, which is directly proportional to the amount of formaldehyde found in samples.
The kit can detect less than 2 µM of formaldehyde in a variety of samples.
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Notes
Formaldehyde (HCHO) is the simplest aldehyde and one of the most often used organic compounds in industrial processes due to its high reactivity with a variety of chemicals. Endogenous formaldehyde can be produced in organisms via metabolic processes while exogenous formaldehyde can be absorbed after oral, dermal or inhalation exposure. formaldehyde is highly toxic due to its capacity to covalently bind to macromolecules, such as DNA, and is well known for its neurotoxicological effects. Exposure to relatively high levels of formaldehyde is believed to cause leukemia, nose and nasopharyngeal cancer, etc.; however the epidemiological evidence is unclear. It can also affect memory and learning capacity.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Formaldehyde Assay Buffer 1 x 25ml Formaldehyde Developer 1 vial Formaldehyde Enzyme Mix 1 vial HCHO Standard 1 x 100µl PicoProbe Blue 1 x 0.4ml -
Research areas
Images
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Typical Formaldehyde (HCHO) standard calibration curve using fluorometric reading.
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Measurement of Formaldehyde (HCHO) concentration in human urine from non-diabetic and diabetic donors. Samples were deproteinized using 10 kD Spin Column (ab93349) and diluted 10X. Diluted samples (20 µL) were spiked with known amount of Formaldehyde (300 pmol). Sample preparation and assay was performed following kit protocol.