Anti-ALPK1 antibody [EPR20911-169] - BSA and Azide free (ab280366)
![Anti-ALPK1 antibody [EPR20911-169] - BSA and Azide free (ab280366)](https://www.abcam.com/ps/products/280/ab280366/Images/ab280366-454997-anti-alpk1-antibody-epr20911169-western-blot-human.jpg "Anti-ALPK1 antibody [EPR20911-169] - BSA and Azide free (ab280366)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20911-169] to ALPK1 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-ALPK1 antibody [EPR20911-169] - BSA and Azide free
See all ALPK1 primary antibodies -
Description
Rabbit monoclonal [EPR20911-169] to ALPK1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type 293T, A549 lysates.
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General notes
ab280366 is the carrier-free version of ab236626. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab280366 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20911-169 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ALPK1 antibody [EPR20911-169] - BSA and Azide free (ab280366)All lanes : Anti-ALPK1 antibody [EPR20911-169] (ab236626) at 1/500 dilution
Lane 1 : ALPK1 knockout 293T (human embryonic kidney epithelial cell) whole cell lysate at 10 µg
Lane 2 : Wild-type 293T whole cell lysate at 10 µg
Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes : Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution
Predicted band size: 139 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?This data was developed using ab236626, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The image was kindly provided by the collaborator.
ab236626 Anti-ALPK1 antibody [EPR20911-169] was shown to react with ALPK1 in 293T cells in Western blot. Loss of signal was observed when ALPK1 knockout sample was used. Wild-type and ALPK1 knockout samples were subjected to SDS-PAGE. ab236626 was incubated 1 hour at room temperature at 1/1000 dilution . Blots were developed with Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/5000 dilution for 1 hour at room temperature before imaging. Anti-beta Tubulin was used as loading control.
Exposure time: 5 minutes
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![Anti-ALPK1 antibody [EPR20911-169] - BSA and Azide free (ab280366)](https://www.abcam.com/ps/products/280/ab280366/Images/ab280366-455240-benefits-of-recombinant-antibodies.jpg)
Anti-ALPK1 antibody [EPR20911-169] - BSA and Azide free (ab280366)