Anti-eIF4E antibody (ab235191)
Key features and details
- Chicken polyclonal to eIF4E
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgY
Overview
-
Product name
Anti-eIF4E antibody
See all eIF4E primary antibodies -
Description
Chicken polyclonal to eIF4E -
Host species
Chicken -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WG: MOLT4, Daudi and HeLa whole cell lysate. IHC-P: Human lung adenocarcinoma tissue. Human cerebellum tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.03% Sodium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
IgY fraction -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4E antibody (ab235191)
Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for eIF4E using ab235191 at 1/1000 in immunohistochemistry. Counterstained with hematoxylin. HIER performed.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4E antibody (ab235191)Formalin-fixed, paraffin-embedded human cerebellum tissue stained for eIF4E using ab235191 at 1/100 for 30mins at RT in immunohistochemistry. Counterstained with hematoxylin, DAB staining. HIER performed using citrate buffer 20mins.
-
Western blot - Anti-eIF4E antibody (ab235191)All lanes : Anti-eIF4E antibody (ab235191) at 5 µg/ml
Lane 1 : MOLT4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 2 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Goat anti-Chicken IgG HRP at 1/40000 dilution
Predicted band size: 25 kDa
Exposure time: 60 seconds
