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Microbiology Organism Bacteria Gram negative Bacteria Escherichia coli

Recombinant E. coli groEL protein (ab113176)

Recombinant <em>E. coli </em> groEL protein (ab113176)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Suitable for: WB, EIA, Functional Studies, ELISA

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Description

  • Product name

    Recombinant E. coli groEL protein
    See all groEL proteins and peptides
  • Purity

    > 90 % SDS-PAGE.
    ab113176 is purified by multi-step chromatography.
  • Expression system

    Escherichia coli
  • Accession

    P0A6F6
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Escherichia coli
    • Sequence

      AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITK DGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIIT EGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTIS ANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLS PYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIA EDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVI SEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQ QIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALH ATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPL RQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTR SALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM
    • Predicted molecular weight

      57 kDa
    • Amino acids

      2 to 548
    • Additional sequence information

      Strain K12.

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Preservative: 0.05% Sodium azide
    Constituents: 0.58% Sodium chloride, 0.79% Tris HCl, 0.08% DTT, 0.1% Magnesium chloride

Images

  • SDS-PAGE - Recombinant E. coli groEL protein (ab113176)
    SDS-PAGE - Recombinant E. coli groEL protein (ab113176)

    SDS-PAGE analysis of ab113176.
    Lane 1: MW marker
    Lane 2: 0.5 μg of ab113176 (groEL protein)
    Lane 3: 1 μg of ab113176 (groEL protein)
    Lane 4: 2 μg of ab113176 (groEL protein)
    Lane 5: 5 μg of ab113176 (groEL protein)
    Detected by Coomassie stain.

  • Western blot - Recombinant E. coli groEL protein (ab113176)
    Western blot - Recombinant E. coli groEL protein (ab113176)

    Western blot analysis using ab113176.
    Lane 1: MW marker
    Lane 2: 100 ng of Recombinant E. coli groEL protein (ab113176) probed with an anti-groEL monoclonal antibody.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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