Recombinant E. coli groEL protein (ab113176)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: WB, EIA, Functional Studies, ELISA
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Product name
Recombinant E. coli groEL protein
See all groEL proteins and peptides -
Purity
> 90 % SDS-PAGE.
ab113176 is purified by multi-step chromatography. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli -
Sequence
AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITK DGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIIT EGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTIS ANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLS PYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIA EDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVI SEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQ QIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALH ATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPL RQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTR SALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM -
Predicted molecular weight
57 kDa -
Amino acids
2 to 548 -
Additional sequence information
Strain K12.
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Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Preservative: 0.05% Sodium azide
Constituents: 0.58% Sodium chloride, 0.79% Tris HCl, 0.08% DTT, 0.1% Magnesium chloride
Images
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SDS-PAGE analysis of ab113176.
Lane 1: MW marker
Lane 2: 0.5 μg of ab113176 (groEL protein)
Lane 3: 1 μg of ab113176 (groEL protein)
Lane 4: 2 μg of ab113176 (groEL protein)
Lane 5: 5 μg of ab113176 (groEL protein)
Detected by Coomassie stain. -
Western blot analysis using ab113176.
Lane 1: MW marker
Lane 2: 100 ng of Recombinant E. coli groEL protein (ab113176) probed with an anti-groEL monoclonal antibody.