Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal
    See all Somatostatin Receptor 2 primary antibodies

  • Description

    Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - C-terminal

  • Host species

    Rabbit

  • Specificity

    The specificity of ab134152 was demonstrated in a Somatostatin Receptor 2-deficient mouse model. Refer to Fischer et al.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Somatostatin Receptor 2 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P30874-1
    (Peptide available as ab171899)

  • Positive control

    • WB: Human and mouse brain tissue lysate. U937, INS-1 and Somatostatin Receptor 2 transfected HEK-293 cell lysates. IHC-P: Human pancreas and brain tissue.

  • General notes

      

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)

    Immunohistochemical analysis of paraffin embedded human brain tissue labeled with ab134152 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    All lanes : Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution

    Lane 1 : U937 (Human histiocytic lymphoma monocyte) whole cell lysates prepared in RIPA lysis method
    Lane 2 : U937 (Human histiocytic lymphoma monocyte) whole cell lysates prepared in 1% SDS hot lysis method
    Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
    Lane 4 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in 1% SDS hot lysis method
    Lane 5 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
    Lane 6 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in 1% SDS hot lysis method
    Lane 7 : A549 (Human lung carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 41 kDa
    Observed band size: 73 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST.

    This antibody doesn’t work in MCF-7 and MDA-MB-231 cells which should be positive (PMID: 16519802). A549 cell is Somatostatin Receptor 2 negative cell line (PMID: 19148511).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) Yuan et al Sci Rep. 2017; 7: 45617. Published online 2017 Apr 5. doi: 10.1038/srep45617

    Immunohistochemical analysis of paraffin-embedded human cervical cancer xenograft tissue (cells derived from liver metastases) using ab134152 at 1/100 dilution.

    Somatostatin Receptor 2 is detected using ab134152.

    From Figure 1 of Yuan et al.

    Yuan et al Sci Rep. 2017; 7: 45617. Published online 2017 Apr 5. doi: 10.1038/srep45617

    Reproduced under the Creative Commons Licence http://creativecommons.org/licenses/by/4.0/

  • Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    All lanes : Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution

    Lane 1 : Human brain lysates prepared in RIPA lysis method
    Lane 2 : Human brain lysates prepared in 1% SDS hot lysis method
    Lane 3 : Mouse brain lysates prepared in RIPA lysis method
    Lane 4 : Mouse brain lysates prepared in 1% SDS hot lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 41 kDa
    Observed band size: 73 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time:
    Lane 1 and 2: 1 second.
    Lane 3 and 4: 5 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)

    Immunohistochemical analysis of paraffin embedded human pancreas tissue labeled with ab134152 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    All lanes : Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution

    Lane 1 : U937 (Human histiocytic lymphoma cell line) cell lysates
    Lane 2 : INS-1 cell lysates
    Lane 3 : Somatostatin Receptor 2 transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 41 kDa

  • Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)

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