Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸ 10 µl 99 841 ₸

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Anti-ATP5L2 + ATP5L antibody [EPR15636] (ab191417)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15636] to ATP5L2 + ATP5L
Suitable for: ICC/IF, IP, WB, IHC-P
Reacts with: Human

Product name

Anti-ATP5L2 + ATP5L antibody [EPR15636]
See all ATP5L2 + ATP5L primary antibodies
Description

Rabbit monoclonal [EPR15636] to ATP5L2 + ATP5L
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, Human fetal liver and Human pancreas lysates; Human kidney tissue; HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15636
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-ATP5L2 + ATP5L antibody [EPR15636] (ab191417)

Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling ATP5L2 + ATP5L with ab191417 at 1/100 dilution (19μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls: anti-ATP5L2 at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.
Western blot - Anti-ATP5L2 + ATP5L antibody [EPR15636] (ab191417)

All lanes : Anti-ATP5L2 + ATP5L antibody [EPR15636] (ab191417) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : Human pancreas tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 11 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5L2 + ATP5L antibody [EPR15636] (ab191417)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ATP5L2 + ATP5L with ab191417 at 1/100 dilution (19.4 μg/ml) followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Inset: Negative control: using PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-ATP5L2 + ATP5L antibody [EPR15636] (ab191417)

Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab191417 at 1/100 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Anti-ATP5L2 + ATP5L antibody [EPR15636] (ab191417)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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