Anti-GABARAPL3 antibody - C-terminal (ab240909)
Key features and details
- Rabbit polyclonal to GABARAPL3 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GABARAPL3 antibody - C-terminal -
Description
Rabbit polyclonal to GABARAPL3 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Rat -
Immunogen
Synthetic peptide corresponding to Human GABARAPL3 (C terminal).
Database link: Q9BY60 -
Positive control
- WB: Rat liver lysate. ICC/IF: SK-N-BE cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-GABARAPL3 antibody - C-terminal (ab240909) at 1/1000 dilution (60 minutes at RT) + Rat liver lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG: HRP for 60 minutes at RT at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 14 kDa
Exposure time: 6 minutesBlock: 5% Skimmed milk in 1X TBST.
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Immunocytochemistry/Immunofluorescence analysis of SK-N-BE (human neuroblastoma cell line) cells labeling GABARAPL3 using ab240909 at 1/100 dilution for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 dilution for 60 minutes at RT.
Counterstains: Phalloidin Texas Red F-Actin stain (red); DAPI nuclear stain (blue).
(A) DAPI nuclear stain (blue) (B) Phalloidin Texas Red F-Actin stain (C) ab240909 (D) Composite.