Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12965] to AP3M2 - N-terminal
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-AP3M2 antibody [EPR12965] - N-terminal
    See all AP3M2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12965] to AP3M2 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human AP3M2 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P53677

  • Positive control

    • Human fetal brain, HepG2, SH-SY5Y and U87-MG lysates; HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)
    Western blot - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)
    All lanes : Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138) at 1/20000 dilution

    Lane 1 : Human fetal brain Lysate
    Lane 2 : HepG2 cell Lysate
    Lane 3 : SH-SY5Y cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)
    Immunocytochemistry/ Immunofluorescence - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling AP3M2 with ab180138 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(Dylight 488) at 1/250 dilution. Conterstained with Dapi blue.

  • Flow Cytometry - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)
    Flow Cytometry - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)

    Flow cytometric analysis of 2% paraformaldehyde fixed U87-MG cells labeling AP3M2 with ab180138 at 1/30 dilution. Secondary antibody Goat anti rabbit IgG (FITC) at 1/75 dilution. Isotype control Rabbit IgG.

  • Western blot - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)
    Western blot - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)
    Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138) at 1/5000 dilution + U87-MG at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 47 kDa

  • Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)
    Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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