Anti-CALML5 antibody [158] - BSA and Azide free (ab276058)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [158] to CALML5 - BSA and Azide free
- Suitable for: Flow Cyt, ICC
- Reacts with: Human
Overview
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Product name
Anti-CALML5 antibody [158] - BSA and Azide free -
Description
Rabbit monoclonal [158] to CALML5 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC Human -
Immunogen
Recombinant fragment corresponding to Human CALML5 aa 1-146.
Database link: Q9NZT1 -
Positive control
- Flow cyt: A431 cells. ICC: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
158 -
Isotype
IgG
Images
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Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cells labeling CALML5 with ab276058, followed by FITC-conjugated secondary antibody (black line) compared with a negative control (grey line). The cells were treated according to manufacturer's manual. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Immunofluorescent analysis of 4% PFA fixed, 0.1% Triton X-100 in PBS permeabilzed A431 (human epidermoid carcinoma cell line) cells labeling CALML5 with ab276058 at 1/60 dilution, at 4°C overnight, followed by Goat Anti-rabbit IgG Alexa Fluor 488-conjugated secondary antibody (green) and counterstained with DAPI (blue). Cells were blocked with 10% serum prior incubation.
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