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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661)

Price and availability

314 937 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21953-74] to Mitofusin 1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat

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Overview

  • Product name

    Anti-Mitofusin 1 antibody [EPR21953-74]
    See all Mitofusin 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR21953-74] to Mitofusin 1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain and heart lysates; Rat brain and heart lysates; NIH/3T3,C2C12 and Neuro-2a whole cell lysates. IP: NIH/3T3 whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21953-74
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • GTP
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitophagy fission and fusion

Images

  • Western blot - Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661)
    Western blot - Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661)
    All lanes : Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661) at 1/1000 dilution

    Lane 1 : Mouse brain lysate at 20 µg
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg
    Lane 3 : Mouse heart lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 84 kDa
    Observed band size: 84 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Eposure times.

    Lane 1: 3 minutes. Lanes 2 & 3: 48 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 25574749).

  • Immunoprecipitation - Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661)
    Immunoprecipitation - Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661)

    Mitofusin 1 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab221661 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221661 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10 μg (Input).
    Lane 2: ab221661 IP in NIH/3T3 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221661 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

  • Western blot - Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661)
    Western blot - Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661)
    All lanes : Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661) at 1/1000 dilution

    Lane 1 : C2C12 (mouse myoblast cell line) whole cell lysate
    Lane 2 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 84 kDa
    Observed band size: 84 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1-3: 3 minutes. Lane 4: 81 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 25574749).

  • Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661)
    Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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