Anti-53BP1 antibody (ab36823)
Key features and details
- Rabbit polyclonal to 53BP1
- Suitable for: Flow Cyt, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-53BP1 antibody
See all 53BP1 primary antibodies -
Description
Rabbit polyclonal to 53BP1 -
Host species
Rabbit -
Specificity
This antibody is specific for 53BP1. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P MouseWB Human
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Immunogen
Synthetic peptide representing a portion of human 53BP1 encoded in part by exons 11 and 12 (LocusLink ID 7158).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (ab36823)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling 53BP1 with ab36823 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-53BP1 antibody (ab36823)All lanes : Anti-53BP1 antibody (ab36823) at 1/1000 dilution
Lane 1 : U2OS cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Predicted band size: 214 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-53BP1 antibody (ab36823)Ab36823, at a dilution of 1/100, staining 53BP1 foci in proliferating MEFs, both normal and exposed to 10 Gy of IR and double color immunofluorescence. Staining was for 2 hours. -
Immunocytochemistry/ Immunofluorescence - Anti-53BP1 antibody (ab36823)Ab36823, at a dilution of 1/100, staining 53BP1 foci in proliferating MO59K cells, both normal and exposed to 10 Gy of IR and double color immunofluorescence. Staining was for 2 hours. -
Flow Cytometry - Anti-53BP1 antibody (ab36823)Flow Cytometry: 53BP1 Antibody [ab36823] - Flow cytometric detection of 53bp1. 1 million Jurkat cells were fixed, permeabilized, and stained with 1.5 mcg/ml anti-53bp1 ab36823 in a 150 mcl reaction. Isotype control (black), anti-53bp1 (red).
