Anti-53BP1 antibody [BL-250-1H11] - BSA free (ab272067)
![Anti-53BP1 antibody [BL-250-1H11] - BSA free (ab272067)](https://www.abcam.com/ps/products/272/ab272067/Images/ab272067-1-ab243868334261anti53bp1antibodybl2501h11immunoprecipitation.jpg "Anti-53BP1 antibody [BL-250-1H11] - BSA free (ab272067)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-250-1H11] to 53BP1 - BSA free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-53BP1 antibody [BL-250-1H11] - BSA free
See all 53BP1 primary antibodies -
Description
Rabbit monoclonal [BL-250-1H11] to 53BP1 - BSA free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human 53BP1 aa 350-400. The exact sequence is proprietary. NP_005648.1 and Gene ID 7158.
Database link: Q12888 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
ab272067 is the carrier-free version of ab243868. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BL-250-1H11 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-53BP1 antibody [BL-250-1H11] - BSA free (ab272067)
53BP1 was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243868 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243868 at 1/1000 dilution.
Lane 1: ab243868 IP in HEK-293T cell lysate.
Lane 2: Contol IgG in HEK-293T cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243868).
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![Anti-53BP1 antibody [BL-250-1H11] - BSA free (ab272067)](https://www.abcam.com/ps/products/272/ab272067/Images/ab272067-2-benefits-of-recombinant-antibodies.png)
Anti-53BP1 antibody [BL-250-1H11] - BSA free (ab272067)
