PE Anti-Vimentin antibody [VI-RE/1] (ab49918)
![PE Anti-Vimentin antibody [VI-RE/1] (ab49918)](https://www.abcam.com/ps/products/49/ab49918/Images/ab49918-249563-anti-vimentin-antibody-vi-re-1-phycoerythrin-immunofluorescence.jpg "PE Anti-Vimentin antibody [VI-RE/1] (ab49918)")
Key features and details
- PE Mouse monoclonal [VI-RE/1] to Vimentin
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-Vimentin antibody [VI-RE/1]
See all Vimentin primary antibodies -
Description
PE Mouse monoclonal [VI-RE/1] to Vimentin -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament protein expressed on a wide variety of mesenchymal and mesodermal cell types. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Recombinant human vimentin.
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Positive control
- ICC/IF: HeLa cells
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General notes
Conjugated with R-Phycoerythrin (PE) under optimum conditions.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Purified antibodies – protein A (G) affinity chromatography before conjugation. Conjugated antibodies –site exclusion chromatography. -
Clonality
Monoclonal -
Clone number
VI-RE/1 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)
ab49918 staining Vimentinn in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab49918 at a working concentration of 10μg/ml (shown in red) and ab185031, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
