Antibodies, Proteins, Kits and Reagents for Life Science

Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17120-11] to TNNI1 - BSA and Azide free
Suitable for: WB, IP, IHC-P
Reacts with: Human

Product name

Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free
See all TNNI1 primary antibodies
Description

Rabbit monoclonal [EPR17120-11] to TNNI1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IP, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab251396 is the carrier-free version of ab203515. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251396 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR17120-11
Isotype

IgG
Research areas

Western blot - Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)

All lanes : Anti-TNNI1 antibody [EPR17120-11] (ab203515) at 1/2000 dilution

Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 5 seconds

This data was developed using ab203515, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

TNNI1 is abundantly expressed in the human skeletal muscle and heart.
Western blot - Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)

Anti-TNNI1 antibody [EPR17120-11] (ab203515) at 1/2000 dilution + Human skeletal muscle tissue lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 5 seconds

This data was developed using ab203515, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

TNNI1 is abundantly expressed in the human skeletal muscle and heart.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)

This data was developed using ab203515, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling TNNI1 with ab203515 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on striated Human skeletal muscle tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)

This data was developed using ab203515, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TNNI1 with ab203515 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. No staining on Human kidney tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)

This data was developed using ab203515, the same antibody clone in a different buffer formulation.TNNI1 was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab203515 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203515 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Human fetal heart whole cell lysate 10 µg (Input). Lane 2: ab203515 IP in Human fetal heart whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203515 in Human fetal heart whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 1 second.
Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)

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