Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17120-11] to TNNI1 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free
    See all TNNI1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17120-11] to TNNI1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251396 is the carrier-free version of ab203515. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251396 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)
    Western blot - Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)
    All lanes : Anti-TNNI1 antibody [EPR17120-11] (ab203515) at 1/2000 dilution

    Lane 1 : Human fetal heart tissue lysate
    Lane 2 : Human fetal kidney tissue lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 5 seconds


    This data was developed using ab203515, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    TNNI1 is abundantly expressed in the human skeletal muscle and heart.

  • Western blot - Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)
    Western blot - Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)
    Anti-TNNI1 antibody [EPR17120-11] (ab203515) at 1/2000 dilution + Human skeletal muscle tissue lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 5 seconds


    This data was developed using ab203515, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    TNNI1 is abundantly expressed in the human skeletal muscle and heart.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)
    This data was developed using ab203515, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling TNNI1 with ab203515 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on striated Human skeletal muscle tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)
    This data was developed using ab203515, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TNNI1 with ab203515 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. No staining on Human kidney tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)
    Immunoprecipitation - Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)
    This data was developed using ab203515, the same antibody clone in a different buffer formulation.TNNI1 was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab203515 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203515 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Human fetal heart whole cell lysate 10 µg (Input). Lane 2: ab203515 IP in Human fetal heart whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203515 in Human fetal heart whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 1 second.
  • Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)
    Anti-TNNI1 antibody [EPR17120-11] - BSA and Azide free (ab251396)

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