Anti-Von Willebrand Factor antibody [EPR18569-105] - BSA and Azide free (ab228523)
![Anti-Von Willebrand Factor antibody [EPR18569-105] - BSA and Azide free (ab228523)](https://www.abcam.com/ps/products/228/ab228523/Images/ab228523-293403-189500wb.jpg "Anti-Von Willebrand Factor antibody [EPR18569-105] - BSA and Azide free (ab228523)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18569-105] to Von Willebrand Factor - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Von Willebrand Factor antibody [EPR18569-105] - BSA and Azide free
See all Von Willebrand Factor primary antibodies -
Description
Rabbit monoclonal [EPR18569-105] to Von Willebrand Factor - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human and mouse plasma and liver lysates; Hepa1-6 whole cell lysate.
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General notes
Ab228523 is the carrier-free version of ab189500. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab228523 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18569-105 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Von Willebrand Factor antibody [EPR18569-105] - BSA and Azide free (ab228523)All lanes : Anti-Von Willebrand Factor antibody [EPR18569-105] (ab189500) at 1/1000 dilution
Lane 1 : Human liver lysate
Lane 2 : Human plasma
Lane 3 : Hep1-6 (mouse hepatoma epithelial cell line) whole cell lysate
Lane 4 : Mouse plasma
Lane 5 : Mouse liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 309 kDa
Observed band size: 309,90 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1,2: 3 minutes; Lane 3: 1 minute; Lane 4,5: 15 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular masses are consistent with what has been described in the literature (PMID: 8204880; PMID: 18344423).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189500).
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![Anti-Von Willebrand Factor antibody [EPR18569-105] - BSA and Azide free (ab228523)](https://www.abcam.com/ps/products/228/ab228523/Images/ab228523-2-benefits-of-recombinant-antibodies.png)
Anti-Von Willebrand Factor antibody [EPR18569-105] - BSA and Azide free (ab228523)
