Anti-TIM 4 antibody [EPR22304-3] - BSA and Azide free (ab254208)
![Anti-TIM 4 antibody [EPR22304-3] - BSA and Azide free (ab254208)](https://www.abcam.com/ps/products/254/ab254208/Images/ab254208-2-222093FC1.jpg "Anti-TIM 4 antibody [EPR22304-3] - BSA and Azide free (ab254208)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22304-3] to TIM 4 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-TIM 4 antibody [EPR22304-3] - BSA and Azide free
See all TIM 4 primary antibodies -
Description
Rabbit monoclonal [EPR22304-3] to TIM 4 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HEK-293T cells. Flow: HEK-293T cells.
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General notes
Ab254208 is the carrier-free version of ab222093. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab254208 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22304-3 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-TIM 4 antibody [EPR22304-3] - BSA and Azide free (ab254208)
Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a myc-tagged TIM 4 expression construct labeling TIM 4 with ab222093 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody, Blue).
Cells were surface stained with ab222093. Then fixed with 2% PFA for 10min, followed by intracellular staining of anti-myc tag conjugated to Alexa Fluor®647. Gated on myc -positive population.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222093).
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![Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR22304-3] - BSA and Azide free (ab254208)](https://www.abcam.com/ps/products/254/ab254208/Images/ab254208-1-222093ICC1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR22304-3] - BSA and Azide free (ab254208)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized TIM 4 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling TIM 4 (Green) with ab222093 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) antibody at 1/1000 dilution. Confocal image showing positive staining in HEK-293T cells transfected with a myc-tagged TIM 4 expression construct. myc-Tag (9B11) Mouse mAb (Alexa Fluor® 594 Conjugate) (ab223894) was used as the counterstain (Red) at 1/100 dilution. The nuclear counterstain was DAPI (Blue).
Negative control: Myc only transfected HEK-293T cells.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222093).
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![Anti-TIM 4 antibody [EPR22304-3] - BSA and Azide free (ab254208)](https://www.abcam.com/ps/products/254/ab254208/Images/ab254208-3-benefits-of-recombinant-antibodies.png)
Anti-TIM 4 antibody [EPR22304-3] - BSA and Azide free (ab254208)
