Human KIM1 ELISA Kit (TIM-1) (ab235081)
Key features and details
- Sensitivity: 1.279 pg/ml
- Range: 7.813 pg/ml - 500 pg/ml
- Sample type: Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human KIM1 ELISA Kit (TIM-1)
See all TIM 1 kits -
Detection method
Colorimetric -
Sample type
Urine -
Assay type
Sandwich (quantitative) -
Sensitivity
1.279 pg/ml -
Range
7.813 pg/ml - 500 pg/ml -
Assay time
2h 0m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The human KIM1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is a complete kit for the quantitative measurement of KIM1 protein in urine.
The ELISA kit is ultra sensitive, reliably measuring as little as 1.2 pg/ml of Kim1. This enables the use of reduced input sample and results in reduced matrix interference. It is high throughput with results in
Kim1 is an early biomarker for kidney injury or disease. It is the most highly upregulated protein in the proximal tubule of the injured kidney. It exists in very low levels in normal kidneys but when upregulated during injury, it is detectable in urine in a wide variety of human diseases. KIM-1 is a potential biomarker for renal injury, which would suggest it has great importance in various kidney diseases and disorders such as chronic kidney disease, as well as acute tubular necrosis and acute kidney failure.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests Assay Buffer 1 x 60ml KIM-1 coated microplate (12x 8 well strips) 1 unit KIM-1 Conjugate 1 x 10ml KIM-1 Detector Antibody 1 x 10ml KIM-1 Standard (25ng/mL) 1 x 0.5ml Plate Sealer 3 units Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml Wash Buffer Concentrate 1 x 100ml -
Research areas
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Function
May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. -
Tissue specificity
Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses. -
Sequence similarities
Belongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD365
- HAVCR
- HAVCR 1
see all -
Database links
- Entrez Gene: 26762 Human
- Omim: 606518 Human
- SwissProt: Q96D42 Human
- Unigene: 129711 Human
Images
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KIM-1 (human) Standard curve