Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-BANK1 antibody [EPR20795] (ab229189)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20795] to BANK1
Suitable for: Flow Cyt, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-BANK1 antibody [EPR20795]
See all BANK1 primary antibodies
Description

Rabbit monoclonal [EPR20795] to BANK1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Rat Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Daudi and Raji whole cell lysates; Human tonsil and fetal spleen lysates. IHC-P: Human tonsil and large B cell lymphoma tissues; Mouse and rat spleen tissues. Flow Cyt: Daudi cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20795
Isotype

IgG
Research areas

Western blot - Anti-BANK1 antibody [EPR20795] (ab229189)

All lanes : Anti-BANK1 antibody [EPR20795] (ab229189) at 1/1000 dilution

Lane 1 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 89 kDa
Observed band size: 100 kDa
why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular mass observed is consistent with the literature (PMID: 11782428).

The Jurkat cell line is a negative control for BANK1.
Western blot - Anti-BANK1 antibody [EPR20795] (ab229189)

All lanes : Anti-BANK1 antibody [EPR20795] (ab229189) at 1/1000 dilution

Lane 1 : Human tonsil lysate
Lane 2 : Human fetal spleen lysate
Lane 3 : Raji (human Burkitt's lymphoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 89 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

Exposure time : Lane 1: 3 minutes; Lane 2: 10 seconds; Lane 3: 58 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

Lane 3 was developed on a BIO-RAD® ChemiDoc™ MP instrument.

The molecular mass observed is consistent with the literature (PMID: 11782428).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20795] (ab229189)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling BANK1 with ab229189 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining is weak in the germinal center but strong in the mantle zones of human tonsil (PMID: 24879116). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20795] (ab229189)

Immunohistochemical analysis of paraffin-embedded human large B cell lymphoma tissue labeling BANK1 with ab229189 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in human large B cell lymphoma cells is observed (PMID: 24879116). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20795] (ab229189)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling BANK1 with ab229189 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in mouse spleen is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20795] (ab229189)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling BANK1 with ab229189 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in rat spleen is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-BANK1 antibody [EPR20795] (ab229189)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) (left panel) and Daudi (human Burkitt's lymphoma cell line) (right panel) cell lines labeling BANK1 with ab229189 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Anti-BANK1 antibody [EPR20795] (ab229189)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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