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Immunology Adaptive Immunity T Cells Non-CD

Anti-FRAT1 antibody [EPR3900] (ab108405)

Price and availability

304 886 ₸

Availability

Order now and get it on Tuesday March 16, 2021

Anti-FRAT1 antibody [EPR3900] (ab108405)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3900] to FRAT1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-FRAT1 antibody [EPR3900]
    See all FRAT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3900] to FRAT1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and MCF7 cell lysates ICC/IF: HeLa cells
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3900
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic

Images

  • Western blot - Anti-FRAT1 antibody [EPR3900] (ab108405)
    Western blot - Anti-FRAT1 antibody [EPR3900] (ab108405)
    All lanes : Anti-FRAT1 antibody [EPR3900] (ab108405) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 29 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-FRAT1 antibody [EPR3900] (ab108405)
    Immunocytochemistry/ Immunofluorescence - Anti-FRAT1 antibody [EPR3900] (ab108405)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling FRAT1 with purified ab108405 at 1/250 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

  • Anti-FRAT1 antibody [EPR3900] (ab108405)
    Anti-FRAT1 antibody [EPR3900] (ab108405)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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