Recombinant mouse CD134 / OX40L receptor protein (Active) (ab221028)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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Functional Studies - Recombinant mouse CD134 / OX40L receptor protein (Active) (ab221028)Immobilized Mouse OX40 Ligand (Fc Tag) at 2 µg/mL (100 µL/well) can bind Mouse OX40 (His Tag) with a linear range of 3.9-31.3 ng/mL (QC tested).
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SDS-PAGE - Recombinant mouse CD134 / OX40L receptor protein (Active) (ab221028)Mouse OX40 (His Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. -
Functional Studies - Recombinant mouse CD134 / OX40L receptor protein (Active) (ab221028)Immobilized ab221028 at 2 μg/mL (100 μl/well) can bind mouse CD134 / OX40 Ligand, Fc Tag with a linear range of 2-30 ng/mL.
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SDS-PAGE - Recombinant mouse CD134 / OX40L receptor protein (Active) (ab221028)SDS-PAGE analysis of reduced ab221028 stained overnight with Coomassie Blue. DTT-reduced protein migrates as 45 kDa in SDS-PAGE due to glycosylation.
