Human IL-17RB ELISA Kit (ab254518)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 64 pg/ml
- Range: 137.5 pg/ml - 8800 pg/ml
- Sample type: Cell culture media, Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-17RB ELISA Kit
See all IL-17RB kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 1.3% Inter-assay Sample n Mean SD CV% Supernatant 3 4.6% -
Sample type
Cell culture supernatant, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
64 pg/ml -
Range
137.5 pg/ml - 8800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 95 % - % Cell culture media 94 % - % -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-17RB ELISA Kit (ab254518) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-17RB protein in cell culture media and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-17RB with 64 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
IL-17RB is a plasma membrane receptor for the proinflammatory cytokines IL-17B and IL-17E. IL-17RB may play a role in controlling the growth and/or differentiation of hematopoietic cells. An alternative spliced secreted variant of IL-17RB has also been identified.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-17RB Capture Antibody 1 x 600µl 10X Human IL-17RB Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human IL-17RB Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Recently, a number of cytokines belonging to the interleukin IL-17 family have been identified. These are termed as IL-17B, IL-17C and IL-17E. IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis, multiple sclerosis, and promotion of tumor growth. IL-17B, C, and E like IL-17 are able to induce proinflammatory responses. However, they do not bind to the IL-17 receptor suggesting that additional IL-17R related receptor might exist. Receptor for IL-17B and IL-17E have been independently isolated and have been designated as EV127 (in mouse) and IL-17RH1 (in human), respectively. IL-17E induces activation of NFkB pathway and like IL-17 also induces production of IL-8. IL-17RH1 mRNA is highly expressed in Kidney and liver and at moderate level in small intestine, colon, and brain. -
Cellular localization
Type I membrane protein (isoform 1). Secreted (isoform 2). -
Alternative names
- CRL 4
- CRL4
- Cytokine receptor CRL4
see all -
Database links
- Entrez Gene: 55540 Human
- Omim: 605458 Human
- SwissProt: Q9NRM6 Human
- Unigene: 5470 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human IL-17RB standard curve in Sample Diluent NS.The IL-17RB standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spike IL-17RB in human cell culture supernatant samples.The concentrations of IL-17RB were measured in duplicates, interpolated from the IL-17RB standard curves and corrected for sample dilution. Undiluted samples are as follows: HepG2 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human IL-17RB ELISA Kit (ab254518)To learn more about the advantages of recombinant antibodies see here.
