Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) (ab221340)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, ELISA
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.4
Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose, 0.87% Sodium chloride, 0.44% L-Arginine
Lyophilized from 0.22 µm filtered solutionThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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ELISA - Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) (ab221340)
Immobilized Recombinant human OX40L/TNFSF4 protein (His tag) (ab221412) at 5 μg/mL (100 μL/well) can bind ab221340 with a linear range of 2-25 ng/mL.
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Functional Studies - Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) (ab221340)FACS assay shows that recombinant Human OX40, Fc Tag can bind to 293T cell overexpressing human OX40L. The concentration of OX40 used is 0.03 µg/ml. -
SDS-PAGE - Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) (ab221340)SDS PAGE analysis of ab221340. The gel was stained overnight with Coomassie Blue. Due to glycosylation, the protein migrates at 65 kDa under reducing conditions.
