Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) (ab221340)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, ELISA
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.4
Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose, 0.87% Sodium chloride, 0.44% L-Arginine
Lyophilized from 0.22 µm filtered solutionThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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Immobilized Recombinant human OX40L/TNFSF4 protein (His tag) (ab221412) at 5 μg/mL (100 μL/well) can bind ab221340 with a linear range of 2-25 ng/mL.
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FACS assay shows that recombinant Human OX40, Fc Tag can bind to 293T cell overexpressing human OX40L. The concentration of OX40 used is 0.03 µg/ml.
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SDS PAGE analysis of ab221340. The gel was stained overnight with Coomassie Blue. Due to glycosylation, the protein migrates at 65 kDa under reducing conditions.