Anti-CD3 antibody [KT3] (ab33429)
Key features and details
- Rat monoclonal [KT3] to CD3
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-CD3 antibody [KT3]
See all CD3 primary antibodies -
Description
Rat monoclonal [KT3] to CD3 -
Host species
Rat -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue/ cell preparation mature T cells (Mouse)
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General notes
ab33429 may be used to trigger proliferation and cytotoxicity of CD3 positive cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Primary antibody notes
ab33429 may be used to trigger proliferation and cytotoxicity of CD3 positive cells. -
Clonality
Monoclonal -
Clone number
KT3 -
Myeloma
NS0 -
Isotype
IgG2a -
Research areas
Images
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ab33429 staining CD3 in mouse spleen tissue by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/250 dilution for 12 hours at 4°C. A goat anti-rat IgG conjugated to Alexa Fluor®488 was used as secondary antibody at a 1/250 dilution.