Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (ab246799)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP162] to CD3 - Low endotoxin, Azide free
  • Suitable for: IHC-P, WB, Flow Cyt (Intra), ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CD3 antibody [SP162] - Low endotoxin, Azide free
    See all CD3 primary antibodies

  • Description

    Rabbit monoclonal [SP162] to CD3 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt (Intra), ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue. Flow Cyt (intra): Jurkat and Mouse Splenocyte cells WB: Jurkat whole cell lysate (ab7899). ICC/IF: Jurkat and EL4.IL-2 cells

  • General notes

    ab246799 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (ab246799)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (ab246799)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD3 with ab135372 at a 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab135372).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (ab246799)
    Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (ab246799)

    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling CD3 with purified ab135372 at 1/10 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab135372).

  • Flow Cytometry - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (ab246799)
    Flow Cytometry - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (ab246799)

    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD3 with purified ab135372 at 1/200 dilution (0.60 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab135372).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (ab246799)
    Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (ab246799)

    Immunocytochemistry/ Immunofluorescence analysis of EL4.IL-2 (mouse lymphoma T lymphocyte) cells labeling CD3 with purified ab135372 at 1/10 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab135372).

  • Flow Cytometry - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (ab246799)
    Flow Cytometry - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (ab246799)

    Flow Cytometry analysis of Mouse Splenocyte cells labeling CD3 with purified ab135372 at 1/20 dilution (5.95 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab135372).

  • Flow Cytometry - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (ab246799)
    Flow Cytometry - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (ab246799)

    Flow cytometric analysis of rabbit anti-CD3 (SP162) antibody ab135372 (1/150) in Jurkat (Human T cell leukemia cell line from peripheral blood) cells (green) compared to negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab135372).

  • Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (ab246799)
    Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (ab246799)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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