Human TCblR ELISA Kit (8D6A) (ab213759)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human TCblR coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Human TCblR antibody 1 x 130µl Lyophilized recombinant Human TCblR standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10. Receptor for the cellular uptake of transcobalamin bound cobalamin. -
Tissue specificity
Expressed abundantly on follicular dendritic cells (FDCs). -
Involvement in disease
Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC) [MIM:613646]; also called methylmalonic aciduria due to transcobalamin receptor defect. MMATC is a metabolic disorder characterized by increased blood C3-acylcarnitine levels, elevated methylmalonate and homocysteine, and low uptake of transcobalamin-bound cobalamin, but normal conversion to adenosylcobalamin and methylcobalamin. Plasma vitamin B12 and total homocysteine are normal. -
Sequence similarities
Contains 2 LDL-receptor class A domains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- 8D6
- 8D6 antigen
- 8D6A
see all -
Database links
- Entrez Gene: 51293 Human
- Omim: 606475 Human
- SwissProt: Q9NPF0 Human
- Unigene: 558499 Human
Images
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Human TCblR ELISA Kit (8D6A) (ab213759) Standard Curve.
Human TCblR ELISA Kit (8D6A) (ab213759) Standard Curve.