Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD3 epsilon antibody [EPR5361(2)] - BSA and Azide free (ab240074)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5361(2)] to CD3 epsilon - BSA and Azide free
  • Suitable for: Flow Cyt, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CD3 epsilon antibody [EPR5361(2)] - BSA and Azide free
    See all CD3 epsilon primary antibodies

  • Description

    Rabbit monoclonal [EPR5361(2)] to CD3 epsilon - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Hut-78 whole cell lysate.

  • General notes

    ab240074 is the carrier-free version of ab133628 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab240074 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-CD3 epsilon antibody [EPR5361(2)] - BSA and Azide free (ab240074)
    Immunoprecipitation - Anti-CD3 epsilon antibody [EPR5361(2)] - BSA and Azide free (ab240074)

    This data was developed using ab133628, the same antibody clone in a different buffer formulation.
    Purified ab133628 at 1/50 dilution (2µg) immunoprecipitating CD3 epsilon in Hut-78 whole cell lysate.
    Lane 1 (input): Hut-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab133628 + Hut-78 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133628 in Hut-78 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 23 kDa

  • Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] - BSA and Azide free (ab240074)
    Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] - BSA and Azide free (ab240074)
    Flow cytometrical analysis of CD3 epsilon in HuT78 cells stained with ab133628 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133628).

  • Anti-CD3 epsilon antibody [EPR5361(2)] - BSA and Azide free (ab240074)
    Anti-CD3 epsilon antibody [EPR5361(2)] - BSA and Azide free (ab240074)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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