Anti-CD28 (phospho Y218) antibody (ab138404)
Key features and details
- Rabbit polyclonal to CD28 (phospho Y218)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD28 (phospho Y218) antibody
See all CD28 primary antibodies -
Description
Rabbit polyclonal to CD28 (phospho Y218) -
Host species
Rabbit -
Specificity
ab138404 detects endogenous levels of CD28 only when phosphorylated at Tyr218. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
A synthetic phosphopeptide derived from Human CD28 around the phosphorylation site of Y218.
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Positive control
- HepG2 cell extracts, treated with nocodazole (1ug/ml, 16 hours).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab138404 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD28 (phospho Y218) antibody (ab138404)Anti-CD28 (phospho Y218) antibody (ab138404) at 1/500 dilution + HepG2 cell extracts, treated with nocodazole (1ug/ml, 16 hours) at 30 µg
Predicted band size: 25 kDa
