Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD3 antibody [SP162] (ab135372)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP162] to CD3
  • Suitable for: ICC/IF, IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CD3 antibody [SP162]
    See all CD3 primary antibodies

  • Description

    Rabbit monoclonal [SP162] to CD3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    ICC/IF
    Mouse
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CD3 aa 150 to the C-terminus (internal sequence). The exact sequence is proprietary.
    Database link: P07766

  • Positive control

    • IHC-P: Human tonsil tissue. Flow Cyt: Jurkat and Mouse Splenocyte cells WB: Jurkat whole cell lysate (ab7899). ICC/IF: Jurkat and EL4.IL-2 cells

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (ab135372)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (ab135372)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD3 with ab135372 at a 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] (ab135372)
    Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] (ab135372)

    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling CD3 with purified ab135372 at 1/10 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)
    Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)

    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD3 with purified ab135372 at 1/200 dilution (0.60 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] (ab135372)
    Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] (ab135372)

    Immunocytochemistry/ Immunofluorescence analysis of EL4.IL-2 (mouse lymphoma T lymphocyte) cells labeling CD3 with purified ab135372 at 1/10 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)
    Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)

    Flow Cytometry analysis of Mouse Splenocyte cells labeling CD3 with purified ab135372 at 1/20 dilution (5.95 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

  • Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)
    Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)

    Flow cytometric analysis of rabbit anti-CD3 (SP162) antibody ab135372 (1/150) in Jurkat (Human T cell leukemia cell line from peripheral blood) cells (green) compared to negative control of rabbit IgG (blue).

  • Western blot - Anti-CD3 antibody [SP162] (ab135372)
    Western blot - Anti-CD3 antibody [SP162] (ab135372)
    Anti-CD3 antibody [SP162] (ab135372) at 1/150 dilution + Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

    Predicted band size: 19 kDa

  • Anti-CD3 antibody [SP162] (ab135372)
    Anti-CD3 antibody [SP162] (ab135372)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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