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PE Anti-CD3 antibody [KT3] (ab22268)

Key features and details

  • PE Rat monoclonal [KT3] to CD3
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG2a

Overview

  • Product name

    PE Anti-CD3 antibody [KT3]
    See all CD3 primary antibodies

  • Description

    PE Rat monoclonal [KT3] to CD3

  • Host species

    Rat

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Specificity

    This antibody recognises the mouse CD3 antigen which is expressed by mature T cells.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    See all applications and species data

  • Immunogen

    Full length native protein (purified) corresponding to Mouse CD3.

  • Positive control

    • Flow Cyt: Mouse spleen, Canine blood leucocytes, Human PBMC

  • General notes

    Purified IgG conjugated to R. Phycoerythrin (RPE).

Properties

Images

  • Flow Cytometry - PE Anti-CD3 antibody [KT3] (ab22268)
    Flow Cytometry - PE Anti-CD3 antibody [KT3] (ab22268)
    ab22268 staining CD3 in mouse spleen cells by Flow Cytometry analysis.
  • Flow Cytometry - PE Anti-CD3 antibody [KT3] (ab22268)
    Flow Cytometry - PE Anti-CD3 antibody [KT3] (ab22268) Image courtesy of an anonymous Abreview.
    ab22268 used in Flow Cytometry.
    Canine blood leucocytes were harvested in PBS, 2% BSA, 0.01% sodium azide after red blood cell lysis, then fixed with paraformaldehyde. ab22268 used at a 1/100 dilution for 1 hour at 4°C.
    The gating strategy used was for lymphocytes.

    See Abreview

  • Flow Cytometry - PE Anti-CD3 antibody [KT3] (ab22268)
    Flow Cytometry - PE Anti-CD3 antibody [KT3] (ab22268) This image is courtesy of an anonymous Abreview

    Flow cytometry analysis of human peripheral blood mononuclear cells (PBMC) labelling CD3 with ab22268 at a dilution of (1/100 in PBS + 0.5% BSA) for 1 hour at 4°C. Cell separation was achieved by FICOLL medium with PBS + 0.5% BSA.
    Gating Strategy: Lymphocytes

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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