Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Human BTN3A1 protein (Fc Chimera) (ab219670)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Human BTN3A1 protein (Fc Chimera)
    See all BTN3A1 proteins and peptides

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    HEK 293 cells

  • Accession

    O00481

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      QFSVLGPSGPILAMVGEDADLPCHLFPTMSAETMELKWVSSSLRQVVNVY ADGKEVEDRQSAPYRGRTSILRDGITAGKAALRIHNVTASDSGKYLCYFQ DGDFYEKALVELKVAALGSDLHVDVKGYKDGGIHLECRSTGWYPQPQIQW SNNKGENIPTVEAPVVADGVGLYAVAASVIMRGSSGEGVSCTIRSSLLGL EKTASISIADPFFRSAQRWIAALAG

    • Predicted molecular weight

      51 kDa including tags

    • Amino acids

      30 to 254

    • Additional sequence information

      Extracellular domain fused with a human IgG1 Fc tag at the C-terminal.

    • Specifications

    Our Abpromise guarantee covers the use of ab219670 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.4
      Constituents: 0.75% Glycine, 0.61% Tris buffer, 5% Trehalose

      Lyophilized from 0.22 µm filtered solution.
      5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

    • Reconstitution
      Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

    General Info

    • Alternative names

      • BT3A1_HUMAN
      • BTN3A1
      • Butyrophilin subfamily 3 member A1
      • CD277

    • Tissue specificity

      Highly expressed in heart, pancreas and lung, Moderately expressed in placenta, liver and muscle.

    • Sequence similarities

      Belongs to the immunoglobulin superfamily. BTN/MOG family.
      Contains 1 B30.2/SPRY domain.
      Contains 2 Ig-like V-type (immunoglobulin-like) domains.

    • Cellular localization

      Membrane.
    • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human BTN3A1 protein (Fc Chimera) (ab219670)
    SDS-PAGE - Recombinant Human BTN3A1 protein (Fc Chimera) (ab219670)

    ab219670 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions.

    The gel was stained overnight with Coomassie Blue.

    The purity of the protein is greater than 95%.

    As a result of glycosylation, the reducing (R) protein migrates as 55 kDa and the non-reducing (NR) protein migrates as 100-120 kDa.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab219670? Please let us know so that we can cite the reference in this datasheet.

ab219670 has not yet been referenced specifically in any publications.

Preparation and Storage

  • BT3A1_HUMAN
  • BTN3A1
  • Butyrophilin subfamily 3 member A1
  • CD277

Images

  • SDS-PAGE - Recombinant Human BTN3A1 protein (Fc Chimera) (ab219670)
    SDS-PAGE - Recombinant Human BTN3A1 protein (Fc Chimera) (ab219670)

    ab219670 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions.

    The gel was stained overnight with Coomassie Blue.

    The purity of the protein is greater than 95%.

    As a result of glycosylation, the reducing (R) protein migrates as 55 kDa and the non-reducing (NR) protein migrates as 100-120 kDa.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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