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Immunology Adaptive Immunity T Cells CD

Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)

Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 90% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

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Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 95% PBS, 5% Trehalose

    Lyophilized from 0.22 µm filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

Images

  • Functional Studies - Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)
    Functional Studies - Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)

    Loaded Anti-OX40 MAb(Human IgG1) on AHC Biosensor, can bind ab221231 with an affinity constant of 6.03 nM as determined in BLI assay. 

  • Functional Studies - Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)
    Functional Studies - Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)

    Loaded ab221412 on AR2G Biosensor, can bind ab221231 with an affinity constant of 0.15 μM as determined in BLI assay.

  • SDS-PAGE - Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)
    SDS-PAGE - Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)

    ab221231 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

  • Functional Studies - Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)
    Functional Studies - Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)

    The purity of ab221231 was more than 85% and around 32-44 kDa verified by SEC-MALS.

  • Functional Studies - Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)
    Functional Studies - Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)
    Immobilized Human OX40, His Tag at 2 µg/mL (100 µL/well)can bind Mouse OX40 Ligand, Fc Tag with a linear range of 0.5-16 ng/mL.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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