Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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Loaded Anti-OX40 MAb(Human IgG1) on AHC Biosensor, can bind ab221231 with an affinity constant of 6.03 nM as determined in BLI assay.
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Loaded ab221412 on AR2G Biosensor, can bind ab221231 with an affinity constant of 0.15 μM as determined in BLI assay.
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ab221231 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
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The purity of ab221231 was more than 85% and around 32-44 kDa verified by SEC-MALS.
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Immobilized Human OX40, His Tag at 2 µg/mL (100 µL/well)can bind Mouse OX40 Ligand, Fc Tag with a linear range of 0.5-16 ng/mL.