Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP6072(2)] to 5 Lipoxygenase/5-LO
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)]
    See all 5 Lipoxygenase/5-LO primary antibodies

  • Description

    Rabbit monoclonal [EP6072(2)] to 5 Lipoxygenase/5-LO

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    Human
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human 5 Lipoxygenase/5-LO aa 100-200. The exact sequence is proprietary.
    Database link: P09917

  • Positive control

    • Fetal thymus, K562 and HUVEC lysates. Human prostate and Human tonsil tissue. HUVEC cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
    Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
    All lanes : Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755) at 1/1000 dilution

    Lane 1 : Rat heart lysate
    Lane 2 : Rat kidney lysate
    Lane 3 : Rat spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size: 78 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)

    ab169755 staining 5 Lipoxygenase/5-LO in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/100). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)

    Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
    Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)

    ab169755 staining 5 Lipoxygenase/5-LO in the Raw264.7 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/100). ab150077 (1/500) an Alexa Fluor® 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.

  • Flow Cytometry - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
    Flow Cytometry - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)

    ab169755 staining 5 Lipoxygenase/5-LO in HUVEC (human umbilical vein/vascular endothelium) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/130. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (black).

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (blue).

  • Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
    Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
    Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755) at 1/1000 dilution + K562 cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size: 78 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
    Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
    Human fetal thymus at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 78 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
    Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)

    Immunofluorescent analysis of HUVEC cells labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.

  • Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
    Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
    All lanes : Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755) at 1/1000 dilution (unpurified)

    Lane 1 : Fetal thymus lysate
    Lane 2 : K562 lysate
    Lane 3 : HUVEC lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 78 kDa

  • Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
    Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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