Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Staining on hepatocellular carcinoma (PMID: 24994855, PMID: 26583321). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

 

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (ab210702) at 1/5000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 2 : C2C12 (mouse myoblast cell line) whole cell lysate at 20 µg
Lane 3 : Mouse testis lysate at 20 µg
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg
Lane 5 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 59 kDa
Observed band size: 59 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1/2/3: 3 minutes; Lanes 4/5: 3 seconds.

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on stroma of human liver (PMID: 24994855, PMID: 26583321). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunofluorescent analysis of methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

 

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunofluorescent analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLA cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue (left panel) and human gastric paracarcinoma (right panel) labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong cytoplasmic staining on human gastric adenocaricoma, compared with weak cytoplasmic staining on the paired paracarcinoma stomach (PMID: 22012600). Both tissue sections are derived from the same patient sample. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

 

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong cytoplasmic staining on human gastric adenocarcinoma (PMID: 22012600). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

 

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on stroma of mouse liver (PMID: 24994855, PMID: 26583321). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

 

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on rat liver (PMID: 24994855, PMID: 26583321). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

 

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (ab210702) at 1/1000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Rat liver lysate
Lane 3 : Rat spleen lysate
Lane 4 : Mouse spleen lysate
Lane 5 : C6 (rat glial tumor cell line) whole cell lysate
Lane 6 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 7 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 59 kDa
Observed band size: 59 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1/2/3: 3 minutes; Lane 4: 15 seconds; Lanes 5/6: 30 seconds; Lane 7: 15 seconds.

Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/400 (red) compared with an Isotype control rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. 

Glucose 6 Phosphate Dehydrogenase was immunoprecipitated from 0.35mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab210702 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab210702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HeLa whole cell lysate 10 μg (Input).

Lane 2: ab210702 IP in HeLa whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab210702 in HeLa whole cell lysate.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 30 seconds.