Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Thursday March 04, 2021

Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6292] to Glucose 6 Phosphate Dehydrogenase
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292]
See all Glucose 6 Phosphate Dehydrogenase primary antibodies
Description

Rabbit monoclonal [EPR6292] to Glucose 6 Phosphate Dehydrogenase
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to residues in the Human Glucose 6 Phosphate Dehydrogenase protein (UniProt ID: P11413).
Positive control
- WB: Molt 4, A549, MCF7, HeLa, and HepG2 cell lysates
  IHC-P: Human testis tissue
General notes
Cellular localisation: centrosome, cytosol, internal side of plasma membrane, intracellular membrane bounded organelle. There are 3 isoforms produced by alternative splicing.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 4.30 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR6292
Isotype

IgG
Research areas

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)

All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525) at 1/1000 dilution

Lane 1 : Molt 4 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit at 1/2000 dilution

Predicted band size: 59 kDa
Observed band size: 58 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)

Immunohistochemistry analysis of Paraffin Embedded Human testis tissue labelling Glucose 6 Phosphate Dehydrogenase with ab133525 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
OI-RD Scanning - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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