Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6292] to Glucose 6 Phosphate Dehydrogenase
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292]
    See all Glucose 6 Phosphate Dehydrogenase primary antibodies

  • Description

    Rabbit monoclonal [EPR6292] to Glucose 6 Phosphate Dehydrogenase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues in the Human Glucose 6 Phosphate Dehydrogenase protein (UniProt ID: P11413).

  • Positive control

    • WB: Molt 4, A549, MCF7, HeLa, and HepG2 cell lysates
      IHC-P: Human testis tissue

  • General notes

    Cellular localisation: centrosome, cytosol, internal side of plasma membrane, intracellular membrane bounded organelle. There are 3 isoforms produced by alternative splicing.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)
    Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)
    All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525) at 1/1000 dilution

    Lane 1 : Molt 4 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit at 1/2000 dilution

    Predicted band size: 59 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)

    Immunohistochemistry analysis of Paraffin Embedded Human testis tissue labelling Glucose 6 Phosphate Dehydrogenase with ab133525 at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)
    OI-RD Scanning - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)
    Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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