Pyruvate dehydrogenase (PDH) WB Antibody Cocktail (ab110416)
Key features and details
- Assay type: Quantitative
Overview
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Product name
Pyruvate dehydrogenase (PDH) WB Antibody Cocktail -
Assay type
Quantitative -
Species reactivity
Reacts with: Cow, Human -
Product overview
ab110416 (MSP02) contains a total of 150 µg of monoclonal antibodies reactive against PDH subunits. Isolated mitochondria are available from several species for use as control samples.
The PDH Western blotting kit contains four different mAbs reacting specifically with E1 alpha, E1 beta, E2 and E2/E3bp. A mAb against the OSCP subunit of ATP synthase is included as a control. This mAb mixture can be used to probe the isolated pyruvate dehydrogenase for integrity and purity.
Cocktail Antibodies:
Mouse anti C-V-OSCP [4C11C10D12] monoclonal:
Amount: 12.5 µg
Working concentration: 0.5 µg/mlMouse anti PDH-E1-alpha [9H9AF5] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/mlMouse anti PDH-E1-beta [17A5E2H8] monoclonal:
Amount: 62.5 µg
Working concentration: 2.5 µg/mlMouse anti PDH-E2 [15D3G9C11] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/mlMouse anti PDH-E2/E3bp [13G2AE2BH5] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/ml -
Tested applications
Suitable for: WBmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 150 µg 300 µg Cocktail of 5 Antibodies 1 x 150µg 1 x 300µg -
Research areas
Images
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Western blot - MitoProfile® Pyruvate dehydrogenase (PDH) WB Antibody Cocktail (ab110416)
Blocked for overnight with 5% Milk/PBS at 4ºC (or 3 hrs at room temperature).
Primary antibody incubatedat 6 µg/mL in 1% milk/PBS for 2hrs at room temperature.
Secondary: HRP-conjugated anti-mouse used according to manufacturers instructions.
Detection method: ECL+
All wash steps carried out in PBS + 0.05% Tween-20.