Recombinant Human PSAP protein (ab167924)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% Densitometry
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: WB, ELISA, SDS-PAGE, MS
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C.
Constituents: 99% Phosphate Buffer, 0.43% Sodium chloride
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ReconstitutionAdd 200µl of deionized water to prepare a working stock solution of 0.5 mg/ml and let the lyophilized pellet dissolve completely. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage.
Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Images
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SDS-PAGE - Recombinant Human PSAP protein (ab167924)12% SDS-PAGE analysis of ab167924
Lane 1: reduced and boiled sample, 2.5μg/lane
Lane 2: non-reduced and non-boiled sample, 2.5μg/laneThe ~66 kDa band corresponds to whole PSAP (prosaposin) molecule that consists of four saposin units. The individual saposins naturally cleave off the prosaposin which results in ~15, ~35 and ~50 kDa fragments of mono-, di- and trisaposins, respectively.