Recombinant Human NAGA protein (His tag) (ab222442)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant Human NAGA protein (His tag)
See all NAGA proteins and peptides -
Purity
> 90 % SDS-PAGE.
ab222442 was purified by conventional chromatography techniques. -
Endotoxin level
Expression system
Baculovirus infected insect cellsAccession
Protein length
Full length proteinAnimal free
NoNature
Recombinant-
Species
Human -
Sequence
LDNGLLQTPPMGWLAWERFRCNINCDEDPKNCISEQLFMEMADRMAQDGW RDMGYTYLNIDDCWIGGRDASGRLMPDPKRFPHGIPFLADYVHSLGLKLG IYADMGNFTCMGYPGTTLDKVVQDAQTFAEWKVDMLKLDGCFSTPEERAQ GYPKMAAALNATGRPIAFSCSWPAYEGGLPPRVNYSLLADICNLWRNYDD IQDSWWSVLSILNWFVEHQDILQPVAGPGHWNDPDMLLIGNFGLSLEQSR AQMALWTVLAAPLLMSTDLRTISAQNMDILQNPLMIKINQDPLGIQGRRI HKEKSLIEVYMRPLSNKASALVFFSCRTDMPYRYHSSLGQLNFTGSVIYE AQDVYSGDIISGLRDETNFTVIINPSGVVMWYLYPIKNLEMSQQHHHHHH -
Predicted molecular weight
46 kDa including tags -
Amino acids
18 to 411 -
Tags
His tag C-Terminus -
Additional sequence information
Full length mature chain without signal peptide.
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab222442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 10% Glycerol (glycerin, glycerine), PBS
General Info
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Alternative names
- Acetylgalactosaminidase, alpha N (alpha galactosidase B)
- Alpha galactosidase B
- Alpha N acetylgalactosaminidase
see all -
Relevance
NAGA encodes the lysosomal enzyme alpha N acetylgalactosaminidase, which cleaves alpha N acetylgalactosaminyl moieties from glycoconjugates. Mutations in NAGA have been identified as the cause of Schindler disease types I and II (type II also known as Kanzaki disease). Alpha galactosidase B was first found to be an isoenzyme of alpha galactosidases, but apparently it differs from alpha galactosidase A in substrate specificity and is alpha N acetylgalactosaminidase. -
Cellular localization
Lysosome
Images
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SDS-PAGE - Recombinant Human NAGA protein (His tag) (ab222442)
ab222442 (3 μg) was analysed by 15% SDS-PAGE.
Predicted molecular mass: 45.5 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab222442 has not yet been referenced specifically in any publications.
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 10% Glycerol (glycerin, glycerine), PBS
Images
-
SDS-PAGE - Recombinant Human NAGA protein (His tag) (ab222442)
ab222442 (3 μg) was analysed by 15% SDS-PAGE.
Predicted molecular mass: 45.5 kDa