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Signal Transduction Metabolism Energy Metabolism

Recombinant Human NAGA protein (His tag) (ab222442)

Recombinant Human NAGA protein (His tag) (ab222442)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: Baculovirus infected insect cells
  • Purity: > 90% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE

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Description

  • Product name

    Recombinant Human NAGA protein (His tag)
    See all NAGA proteins and peptides
  • Purity

    > 90 % SDS-PAGE.
    ab222442 was purified by conventional chromatography techniques.
  • Endotoxin level

  • Expression system

    Baculovirus infected insect cells
  • Accession

    P17050
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      LDNGLLQTPPMGWLAWERFRCNINCDEDPKNCISEQLFMEMADRMAQDGW RDMGYTYLNIDDCWIGGRDASGRLMPDPKRFPHGIPFLADYVHSLGLKLG IYADMGNFTCMGYPGTTLDKVVQDAQTFAEWKVDMLKLDGCFSTPEERAQ GYPKMAAALNATGRPIAFSCSWPAYEGGLPPRVNYSLLADICNLWRNYDD IQDSWWSVLSILNWFVEHQDILQPVAGPGHWNDPDMLLIGNFGLSLEQSR AQMALWTVLAAPLLMSTDLRTISAQNMDILQNPLMIKINQDPLGIQGRRI HKEKSLIEVYMRPLSNKASALVFFSCRTDMPYRYHSSLGQLNFTGSVIYE AQDVYSGDIISGLRDETNFTVIINPSGVVMWYLYPIKNLEMSQQHHHHHH
    • Predicted molecular weight

      46 kDa including tags
    • Amino acids

      18 to 411
    • Tags

      His tag C-Terminus
    • Additional sequence information

      Full length mature chain without signal peptide.
  • Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab222442 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituents: 10% Glycerol (glycerin, glycerine), PBS

    General Info

    • Alternative names

      • Acetylgalactosaminidase, alpha N (alpha galactosidase B)
      • Alpha galactosidase B
      • Alpha N acetylgalactosaminidase
      • GALB
      • N acetylgalactosaminidase, alpha
      see all
    • Relevance

      NAGA encodes the lysosomal enzyme alpha N acetylgalactosaminidase, which cleaves alpha N acetylgalactosaminyl moieties from glycoconjugates. Mutations in NAGA have been identified as the cause of Schindler disease types I and II (type II also known as Kanzaki disease). Alpha galactosidase B was first found to be an isoenzyme of alpha galactosidases, but apparently it differs from alpha galactosidase A in substrate specificity and is alpha N acetylgalactosaminidase.
    • Cellular localization

      Lysosome

Images

  • SDS-PAGE - Recombinant Human NAGA protein (His tag) (ab222442)
    SDS-PAGE - Recombinant Human NAGA protein (His tag) (ab222442)

    ab222442 (3 μg) was analysed by 15% SDS-PAGE.

    Predicted molecular mass: 45.5 kDa

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab222442? Please let us know so that we can cite the reference in this datasheet.

    ab222442 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituents: 10% Glycerol (glycerin, glycerine), PBS

    Images

    • SDS-PAGE - Recombinant Human NAGA protein (His tag) (ab222442)
      SDS-PAGE - Recombinant Human NAGA protein (His tag) (ab222442)

      ab222442 (3 μg) was analysed by 15% SDS-PAGE.

      Predicted molecular mass: 45.5 kDa

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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