Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11369(B)] to GLUD1 + GLUD2
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] -
Description
Rabbit monoclonal [EPR11369(B)] to GLUD1 + GLUD2 -
Host species
Rabbit -
Specificity
Ab166618 immunogen shows 100% identity to human GLUD2.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human GLUD1 aa 50-150. The exact sequence is proprietary.
Database link: P00367 -
Positive control
- Human fetal liver, HepG2, HeLa and 293T lysates, Human liver & skeletal muscle tissue, permeabilized 293T cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11369(B) -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
ab166618 showing +ve staining in Human normal tonsil.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618) at 1/10000 dilution
Lane 1 : Human fetal liver lysates
Lane 2 : HepG2 lysates
Lane 3 : HeLa lysates
Lane 4 : 293T lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 52 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
ab166618 showing +ve staining in Human normal colon.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
ab166618 showing +ve staining in Human normal kidney.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
ab166618 showing +ve staining in Human normal brain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GLUD1 with ab166618 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling GLUD1 with ab166618 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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