Anti-SPR antibody [EPR9290] - BSA and Azide free (ab249302)
![Anti-SPR antibody [EPR9290] - BSA and Azide free (ab249302)](https://www.abcam.com/ps/products/249/ab249302/Images/ab249302-384519-anti-spr-antibody-epr9290-bsa-and-azide-free-western-blot-hela-mcf7.jpg "Anti-SPR antibody [EPR9290] - BSA and Azide free (ab249302)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9290] to SPR - BSA and Azide free
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-SPR antibody [EPR9290] - BSA and Azide free
See all SPR primary antibodies -
Description
Rabbit monoclonal [EPR9290] to SPR - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7 and HeLa cell lysates.
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General notes
Ab249302 is the carrier-free version of ab157194. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249302 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9290 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SPR antibody [EPR9290] - BSA and Azide free (ab249302)All lanes : Anti-SPR antibody [EPR9290] (ab157194) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SPR knockout HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab157194).
Lanes 1-3: Merged signal (red and green). Green - ab157194 observed at 26 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab157194 Anti-SPR antibody [EPR9290] was shown to specifically react with SPR in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264681 (knockout cell lysate ab258700) was used. Wild-type and SPR knockout samples were subjected to SDS-PAGE. ab157194 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Anti-SPR antibody [EPR9290] - BSA and Azide free (ab249302)](https://www.abcam.com/ps/products/249/ab249302/Images/ab249302-2-benefits-of-recombinant-antibodies.png)
Anti-SPR antibody [EPR9290] - BSA and Azide free (ab249302)
