Anti-iNOS antibody [NOS-IN] (ab49999)
![Anti-iNOS antibody [NOS-IN] (ab49999)](https://www.abcam.com/ps/products/49/ab49999/Images/ab49999-271866-anti-inos-antibody-nos-in-immunofluorescence.jpg "Anti-iNOS antibody [NOS-IN] (ab49999)")
Key features and details
- Mouse monoclonal [NOS-IN] to iNOS
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-iNOS antibody [NOS-IN]
See all iNOS primary antibodies -
Description
Mouse monoclonal [NOS-IN] to iNOS -
Host species
Mouse -
Specificity
This antibody reacts specifically with inducible-type nitricoxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain.
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Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseWB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse iNOS aa 1126-1144 conjugated to keyhole limpet haemocyanin.
Sequence:FSYGAKKGSALEEPKATRL
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Positive control
- WB: Whole cell extract of RAW 264.7 activated with LPS and IFN-gamma. ICC/IF: RAW 264.7 activated with LPS and IFN-gamma.
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General notes
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.087% Sodium azide
Constituent: 2.93% PBS -
Concentration information loading... -
Purification notes
This antibody is purified from hybridoma cell culture. -
Clonality
Monoclonal -
Clone number
NOS-IN -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [NOS-IN] (ab49999)
Immunocytochemistry/ Immunofluorescence analysis of IFN-γ/LPS-activated RAW-264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling iNOS with ab49999 at 1/2000 dilution.
Cells were fixed and permeabilized with 4% paraformaldehyde and 0.5% Triton™ X-100. The antibody was developed using Goat anti-Mouse IgG, Cy3-conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
This image was generated using the ascites version of the product.
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![Western blot - Anti-iNOS antibody [NOS-IN] (ab49999)](https://www.abcam.com/ps/products/49/ab49999/Images/ab49999-271868-anti-inos-antibody-nos-in-western-blot.jpg)
Western blot - Anti-iNOS antibody [NOS-IN] (ab49999)Lane 1 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/500 dilution
Lane 2 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/1000 dilution
Lane 3 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/2500 dilution
Lane 4 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/5000 dilution
All lanes : IFN-gamma/LPS-activated RAW-264.7 cell extract
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase and chemiluminescent substrate
Predicted band size: 131 kDaThis image was generated using the ascites version of the product.
