Anti-Chk1 (phospho S317) antibody (ab226929)
Key features and details
- Rabbit polyclonal to Chk1 (phospho S317)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Chk1 (phospho S317) antibody
See all Chk1 primary antibodies -
Description
Rabbit polyclonal to Chk1 (phospho S317) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide within Human Chk1 (internal sequence) (phospho S317). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: O14757 -
Positive control
- WB: HCT 116 cells treated with cisplatin. ICC/IF: HeLa cells treated with UVC light.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Chk1 (phospho S317) antibody (ab226929)All lanes : Anti-Chk1 (phospho S317) antibody (ab226929) at 1/500 dilution
Lane 1 : HCT 116 (human colorectal carcinoma cell line) whole cell extract
Lane 2 : HCT 116, treated with 30 uM cisplatin for 24 hours, whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 54 kDa10% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-Chk1 (phospho S317) antibody (ab226929)Left panels: Control 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Chk1 (phospho S317) (green) using ab226929 at 1/500 dilution in ICC/IF.
Right panels: UVC-exposed (100 J/m2 followed by 8 hours recovery) 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Chk1 (phospho S317) (green) using ab226929 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
