Rat IL-1 alpha ELISA Kit (ab235646)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.205 pg/ml
- Range: 5.47 pg/ml - 350 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat IL-1 alpha ELISA Kit
See all IL-1 alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 3 2.7% Inter-assay Sample n Mean SD CV% Supernatant 8 2.9% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.205 pg/ml -
Range
5.47 pg/ml - 350 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 112 101% - 121% Serum 98 96% - 103% Hep Plasma 97 97% - 98% EDTA Plasma 92 90% - 93% Cit plasma 99 96% - 104% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat IL-1 alpha ELISA Kit (ab235646) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-1 alpha protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-1 alpha with 0.205 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat IL-1 alpha Capture Antibody 1 x 600µl 10X Rat IL-1 alpha Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Plate Seals 1 unit Rat IL-1 alpha Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. -
Sequence similarities
Belongs to the IL-1 family. -
Domain
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. -
Cellular localization
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. - Information by UniProt
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Alternative names
- BAF
- FAF
- Hematopoietin 1
see all -
Database links
- Entrez Gene: 24493 Rat
- SwissProt: P16598 Rat
- Unigene: 12300 Rat
Images
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Other - Rat IL-1 alpha ELISA Kit (ab235646)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Rat IL-1 alpha Standard Curve Comparison.Standard Curve comparison between Rat IL-1 alpha SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Interpolated concentrations of native IL-1 alpha in rat cell culture supernatant sample.The concentrations of IL-1 alpha were measured in duplicates, interpolated from the IL-1 alpha standard curves and corrected for sample dilution. Undiluted sample was rat spleen cell supernatant 100%. Cells were treated with 100 ng/mL LPS in RPMI culture media containing glutaMAX and 10% fetal bovine serum over 3 days. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1 alpha concentration was determined to be 251.71 pg/mL in LPS treated spleen cell supernatant 100%.
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Example of rat IL-1 alpha standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Sandwich ELISA - Rat IL-1 alpha ELISA Kit (ab235646)To learn more about the advantages of recombinant antibodies see here.