Recombinant human CD32A protein (ab207140)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
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Images
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Functional Studies - Recombinant human CD32A protein (ab207140)
Immobilized Human CD32a (H167), His Tag (SPR & BLI verified) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 1.45 µM as determined in a SPR assay (Biacore T200).
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SDS-PAGE - Recombinant human CD32A protein (ab207140)Human CD32a (H167), His Tag (SPR & BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Functional Studies - Recombinant human CD32A protein (ab207140)Loaded Human CD32a (H167), His Tag (SPR & BLI verified) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 1.30 µM as determined in BLI assay.
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SDS-PAGE - Recombinant human CD32A protein (ab207140)DTT-reduced SDS-PAGE analysis of ab207140 stained overnight with Coomassie Blue.
DTT-reduced protein migrates as 29-32 kDa in SDS-PAGE due to glycosylation.
