Mouse CD80 ELISA Kit (ab245721)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2 pg/ml
- Range: 6.25 pg/ml - 400 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CD80 ELISA Kit
See all CD80 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 5 5.6% Inter-assay Sample n Mean SD CV% serum 3 5.7% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2 pg/ml -
Range
6.25 pg/ml - 400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 106 100% - 113% Serum 101 91% - 110% Cell culture extracts 99 88% - 106% Hep Plasma 111 108% - 115% Cit plasma 107 99% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse CD80 ELISA Kit (ab245721) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD80 protein in cell culture extracts, cell culture supernatant, cit plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD80 with 2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CD80 Capture Antibody 1 x 600µl 10X Mouse CD80 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse CD80 Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
Tissue specificity
Expressed on activated B-cells, macrophages and dendritic cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Activation B7-1 antigen
- B lymphocyte activation antigen B7
- B7
see all -
Database links
- Entrez Gene: 12519 Mouse
- SwissProt: Q00609 Mouse
- Unigene: 89474 Mouse
Images
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Other - Mouse CD80 ELISA Kit (ab245721)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse CD80 standard curve in Sample Diluent NS.The CD80 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Example of mouse CD80 standard curve in 1X Cell Extraction Buffer PTR.The CD80 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native CD80 in mouse serum, plasma, and cell culture supernatant samples.The concentrations of CD80 were measured in duplicates, interpolated from the CD80 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 50%, plasma (heparin) 25%, and spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 1,505.1 pg/mL in serum, 829.0 pg/mL in plasma (citrate), 1,487.5 pg/mL in plasma (heparin), and 228.8 pg/ml in spleen supernatant. -
Interpolated concentrations of native CD80 in mouse C2C12 cell extract based on a 10 µg/mL extract load.The concentrations of CD80 were measured in duplicate and interpolated from the CD80 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 314.7 pg/mL in C2C12 cell extract. -
Sandwich ELISA - Mouse CD80 ELISA Kit (ab245721)To learn more about the advantages of recombinant antibodies see here.
